bbsi digested px459 vector  (Addgene inc)

Journal: Cell

Article Title: G3BP1 is a tunable switch that triggers phase separation to assemble stress granules

doi: 10.1016/j.cell.2020.03.046

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cytoscape version 3.7.1 ( Shannon et al., 2003 ) was used for the visualization of this network in . . CRISPR-Cas9-Mediated Knockout Cells gRNA for each gene was designed through http://crispr.mit.edu/ and synthesized DNA oligos were ligated into BbsI- (NEB) digested px459 vectors (Addgene, 62988) ( Ran et al., 2013 ).

Techniques: Virus, Recombinant, Protease Inhibitor, Electron Microscopy, Lysis, Extraction, Staining, Isolation, Mutagenesis, Sequencing, Plasmid Preparation, Software, Imaging